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deoxy big chap (dbc)  (Millipore)


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    Structured Review

    Millipore deoxy big chap (dbc)
    Deoxy Big Chap (Dbc), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/deoxy+big+chap/deoxybigchap/pm37578227-219-0-13
    Average 90 stars, based on 1 article reviews
    deoxy big chap (dbc) - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Magnetic Beads:

    Article Title: Regulated Erlin-dependent release of the B12 transmembrane J-protein promotes ER membrane penetration of a non-enveloped virus
    Article Snippet: .. FLAG M2 antibody-conjugated agarose beads, 3xFLAG peptide, Triton X-100 and Brefeldin A (BFA) were purchased from Sigma-Aldrich, PMSF, digitonin, and Deoxy Big Chap from EMD Millipore Chemicals (San Diego, CA), Protein G magnetic beads, Lipofectamine 2000, Lipofectamine RNAi max, and Hygromycin B from Thermo Fisher Scientific, Fugene HD from Promega (Madison, WI), Polyethylenimine HCl MAX, Linear, Mw 40,000 (PEI) from Polysciences Inc. (Warminster, PA), the rapamycin-1 anologue heterodimerizer from Takara Bio Inc. (Kusatsu, Shiga, JAPAN), and Blasticidin S HCl from Corning (Corning, NY). .. CV-1 and HEK 293T cells (ATCC, Manassas, VA) were cultured in DMEM (Thermo Fisher Scientific) containing 10% fetal bovine serum (Corning), 10 U/ml penicillin (Thermo fisher Scientific), and 10 μg/ml streptomycin (Thermo Fisher Scientific).

    Incubation:

    Article Title: The nucleotide exchange factors Grp170 and Sil1 induce cholera toxin release from BiP to enable retrotranslocation
    Article Snippet: .. 293T cells were incubated with or without 10 nM CT in HBSS for 90 min, harvested, and lysed in buffer containing 4-(2-hydroxyethyl)1-piperazineethanesulfonic acid (HEPES; pH 7.5, 50 mM), NaCl (150 mM), sucrose (250 mM), MgCl2 (2 mM), N-ethylmaleimide (10 mM), 1% Triton X-100 or 1% deoxy Big Chap (Sigma-Aldrich), and protease inhibitors for 30 min at 4°C. ..

    other:

    Article Title: The Get1/2 insertase forms a channel to mediate the insertion of tail-anchored proteins into the ER
    Article Snippet: Deoxy Big CHAP , Calbiochem , Cat#86303-23-3.



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    Image Search Results


    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: The Get1/2 insertase forms a channel to mediate the insertion of tail-anchored proteins into the ER

    doi: 10.1016/j.celrep.2022.111921

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Deoxy Big CHAP , Calbiochem , Cat#86303-23-3.

    Techniques: Virus, Recombinant, Software

    Journal: eLife

    Article Title: The architecture of EMC reveals a path for membrane protein insertion

    doi: 10.7554/eLife.57887

    Figure Lengend Snippet:

    Article Snippet: Chemical compound, drug , deoxy big CHAP (DBC) , Anatrace , Cat# 256455 , .

    Techniques: Suspension, CRISPR, Recombinant, Crystallization Assay, Plasmid Preparation, Mutagenesis, Construct, Produced, Sequencing, Purification, In Vitro, Software